Automated Particle Identification through Regression Analysis of Size, Shape and Colour

Rapid point of care diagnostic tests and tests to provide therapeutic information are now available for a range of specific conditions from the measurement of blood glucose levels for diabetes to card agglutination tests for parasitic infections. Due to a lack of specificity these test are often then backed up by more conventional lab based diagnostic methods for example a card agglutination test may be carried out for a suspected parasitic infection in the field and if positive a blood sample can then be sent to a lab for confirmation. The eventual diagnosis is often achieved by microscopic examination of the sample. In this paper we propose a computerized vision system for aiding in the diagnostic process; this system used a novel particle recognition algorithm to improve specificity and speed during the diagnostic process. We will show the detection and classification of different types of cells in a diluted blood sample using regression analysis of their size, shape and colour. The first step is to define the objects to be tracked by a Gaussian Mixture Model for background subtraction and binary opening and closing for noise suppression. After subtracting the objects of interest from the background the next challenge is to predict if a given object belongs to a certain category or not. This is a classification problem, and the output of the algorithm is a Boolean value (true/false). As such the computer program should be able to ”predict” with reasonable level of confidence if a given particle belongs to the kind we are looking for or not. We show the use of a binary logistic regression analysis with three continuous predictors: size, shape and color histogram. The results suggest this variables could be very useful in a logistic regression equation as they proved to have a relatively high predictive value on their own

Being Different Yet Feeling Similar: The Influence Of Demographic Composition And Organizational Culture On Work Processes And Outcomes

Drawing from self-categorization theory, we tested hypotheses on the effects of an organization's demographic composition and cultural emphasis on work processes and outcomes. Using an organizational simulation, we found that the extent to which an organization emphasized individualistic or collectivistic values interacted with demographic composition to influence social interaction, conflict, productivity, and perceptions of creativity among 258 MBA students. Our findings suggest that the purported benefits of demographic diversity are more likely to emerge in organizations that, through their culture, make organizational membership salient and encourage people to categorize one another as having the organization's interests in common, rather than those that emphasize individualism and distinctiveness among members.(.)Managemen

Time resolved spectroscopy of dust and gas from extrasolar planetesimals orbiting WD 1145+017

Multiple long and variable transits caused by dust from possibly disintegrating asteroids were detected in light curves of WD 1145+017. We present time-resolved spectroscopic observations of this target with QUCAM CCDs mounted in the Intermediate dispersion Spectrograph and Imaging System at the 4.2-m William Herschel Telescope in two different spectral arms: the blue arm covering 3800-4025 {\AA} and the red arm covering 7000-7430 {\AA}. When comparing individual transits in both arms, our observations show with 20 {\sigma} significance an evident colour difference between the in- and out-of-transit data of the order of 0.05-0.1 mag, where transits are deeper in the red arm. We also show with > 6 {\sigma} significance that spectral lines in the blue arm are shallower during transits than out-of-transit. For the circumstellar lines it also appears that during transits the reduction in absorption is larger on the red side of the spectral profiles. Our results confirm previous findings showing the u'-band excess and a decrease in line absorption during transits. Both can be explained by an opaque body blocking a fraction of the gas disc causing the absorption, implying that the absorbing gas is between the white dwarf and the transiting objects. Our results also demonstrate the capability of EMCCDs to perform high-quality time resolved spectroscopy of relatively faint targets.Comment: 9 pages, 5 figures. Accepted to MNRA

A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

DNA topoisomerases are required to resolve DNA topological stress. Despite this essential role, abortive topoisomerase activity generates aberrant protein-linked DNA breaks, jeopardising genome stability. Here, to understand the genomic distribution and mechanisms underpinning topoisomerase-induced DNA breaks, we map Top2 DNA cleavage with strand-specific nucleotide resolution across the S. cerevisiae and human genomes—and use the meiotic Spo11 protein to validate the broad applicability of this method to explore the role of diverse topoisomerase family members. Our data characterises Mre11-dependent repair in yeast and defines two strikingly different fractions of Top2 activity in humans: tightly localised CTCF-proximal, and broadly distributed transcription-proximal, the latter correlated with gene length and expression. Moreover, single nucleotide accuracy reveals the influence primary DNA sequence has upon Top2 cleavage—distinguishing sites likely to form canonical DNA double-strand breaks (DSBs) from those predisposed to form strand-biased DNA single-strand breaks (SSBs) induced by etoposide (VP16) in vivo

A ground-based NUV secondary eclipse observation of KELT-9b

KELT-9b is a recently discovered exoplanet with a 1.49 d orbit around a B9.5/A0-type star. The unparalleled levels of UV irradiation it receives from its host star put KELT-9b in its own unique class of ultra-hot Jupiters, with an equilibrium temperature > 4000 K. The high quantities of dissociated hydrogen and atomic metals present in the dayside atmosphere of KELT-9b bear more resemblance to a K-type star than a gas giant. We present a single observation of KELT-9b during its secondary eclipse, taken with the Wide Field Camera on the Isaac Newton Telescope (INT). This observation was taken in the U-band, a window particularly sensitive to Rayleigh scattering. We do not detect a secondary eclipse signal, but our 3$\sigma$ upper limit of 181 ppm on the depth allows us to constrain the dayside temperature of KELT-9b at pressures of ~30 mbar to 4995 K (3$\sigma$). Although we can place an observational constraint of $A_g<$ 0.14, our models suggest that the actual value is considerably lower than this due to H$^-$ opacity. This places KELT-9b squarely in the albedo regime populated by its cooler cousins, almost all of which reflect very small components of the light incident on their daysides. This work demonstrates the ability of ground-based 2m-class telescopes like the INT to perform secondary eclipse studies in the NUV, which have previously only been conducted from space-based facilities.Comment: Accepted in ApJL. 7 pages, 3 figure

Expanding known dinoflagellate distributions : investigations of slurry cultures from Caspian Sea sediment

To investigate the disparity between plankton and cyst records, sediment slurry cultures were used to isolate the motile stage of dinoflagellates from Caspian Sea sediment. This has resulted in new records for this area of Kryptoperidinium foliaceum, Gymnodinium aureolum and Woloszynskia sp. and for the cyst record, Scrippsiella acuminata. Two Gonyaulax species were isolated, one was identified as Gonyaulax baltica and the other an unknown species. Cultures of Lingulodinium polyedra were also isolated. The approach of using slurries was useful to provide cultures from sediments that were relatively poor in dinoflagellate cysts with contents

Positive regulation of meiotic DNA double-strand break formation by activation of the DNA damage checkpoint kinase Mec1(ATR)

During meiosis, formation and repair of programmed DNA double-strand breaks (DSBs) create genetic exchange between homologous chromosomes-a process that is critical for reductional meiotic chromosome segregation and the production of genetically diverse sexually reproducing populations. Meiotic DSB formation is a complex process, requiring numerous proteins, of which Spo11 is the evolutionarily conserved catalytic subunit. Precisely how Spo11 and its accessory proteins function or are regulated is unclear. Here, we use Saccharomyces cerevisiae to reveal that meiotic DSB formation is modulated by the Mec1(ATR) branch of the DNA damage signalling cascade, promoting DSB formation when Spo11-mediated catalysis is compromised. Activation of the positive feedback pathway correlates with the formation of single-stranded DNA (ssDNA) recombination intermediates and activation of the downstream kinase, Mek1. We show that the requirement for checkpoint activation can be rescued by prolonging meiotic prophase by deleting the NDT80 transcription factor, and that even transient prophase arrest caused by Ndt80 depletion is sufficient to restore meiotic spore viability in checkpoint mutants. Our observations are unexpected given recent reports that the complementary kinase pathway Tel1(ATM) acts to inhibit DSB formation. We propose that such antagonistic regulation of DSB formation by Mec1 and Tel1 creates a regulatory mechanism, where the absolute frequency of DSBs is maintained at a level optimal for genetic exchange and efficient chromosome segregation